Anti-CD3 antibody is used in proliferating T lymphocytes. Among many clones, the clone "OKT3" is most popularly used. Anti-CD3 mAb GMP grade is manufactured as a quality- assured product, according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products. No animal- or human- derived components were used for manufacture of this product. This product can be used for ex vivo cell culture processing and is limited to investigational use only.

· GMP grade anti-CD3 monoclonal antibody (clone; OKT3)

· To be used in T lymphocyte proliferation
· No animal- or human- derived components were used for manufacture of this product
· For research use and ex vivo cell culture processing only

[Product]

   anti-CD3 monoclonal antibody Clone" OKT3"( isotype: mouse lgG2a)
[Size]
    1.0mg protein/1.0ml in a 2ml cryovial
[Storage Buffer]
    Phosphate- buffered saline( PBS), pH7.4, with 200 ppm polysorbate 80.
[Quality]
    -Includes no animal or human derived material.
    -Endotoxin; <5 EU/ml as determined by kinetic Limulus Amebocyte Lysate (LAL) assay.
    -Manufactured according to relevant GMP guidelines.
[Shipping and Storage]
    2-8℃
[Application]
    For research use and ex vivo cell culture processing only. Not intended for human in vivo applications. Do not inject or infuse this product directly into a patient.
[Application 1]
CIK ( Cytokine- induced Killer) cells expansion by stimulation with Anti-CD3 mAb GMP grade and cytokine.

PBMCs were stimulated with Anti-CD3 mAb GMP grade, IFN-γ, IL-1α and IL-2 for 5 days. After stimulation, activated lymphocyte were diluted with medium and cultivated for 9 days. Figure shows expansion fold on day 5,7,9,12 and 14.
[Application2]
Co-stimulatory effect of Anti-CD3 mAb GMP grade and Retronectin in T-lymphocyte expansion.

PBMCs were stimulated with pre-coated Anti- CD3 mAb GMP grade or pre- coated Anti- CD3 mAb GMP grade and Retronectin for 4 days. After stimulation, activated lymphocyte were diluted with GT-T551 medium and cultivated. Figure shows expansion fold on day10.